High performance liquid chromatographic determination of peptidase activity toward proline-containing peptides

This review presents a simple and highly reproducible method for the determination of proline-containing peptides by high performance liquid chromatography (HPLC). Since proline residues exert unique structural constraints on peptide chains, proline-specific peptidases are required for the hydrolysis of peptides containing this imino acid. The following peptidases are discussed: X-Pro amino peptidase, X-Pro dipeptidase, dipeptidyl peptidase IV, lysosomal Pro-X carboxypeptidase, membrane Pro-X carboxypeptidase, dipeptidyl peptidase II, prolyl oligopeptidase, and prolyl iminopeptidase.