A simple capillary zone electrophoretic assay for reserpin using end-column amperometric detection at a carbon fiber micro-disk array electrode Original Research Article

Capillary zone electrophoresis was employed for the determination of reserpin using an end-column amperometric detection with a carbon fiber micro-disk array electrode at a constant potential of +1.30 V vs. saturated calomel electrode. The optimum conditions of separation and detection are 1.0 × 10−4 mol/l Tris–8.4 × 10−5 mol/l HCl for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 8.0 × 10−7 mol/l or 1.4 fmol (S/N = 2). The relative standard deviation is 2.5% for the migration time and 1.5% for the electrophoretic peak current. The method was applied to the determination of reserpin in human serum.