Title of article :
Spectrophotometric enzymatic cycling method using l-glutamate dehydrogenase and d-phenylglycine aminotransferase for determination of l-glutamate in foods Original Research Article
Author/Authors :
Wanida Khampha، نويسنده , , Vithaya Meevootisom، نويسنده , , Suthep Wiyakrutta، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Abstract :
This report describes a new spectrophotometric method capable of determining low levels of l-glutamate. The assay is based on substrate cycling between l-glutamate dehydrogenase (GlDH) and the novel enzyme d-phenylglycine aminotransferase (d-PhgAT). In this system, GlDH converts l-glutamate to 2-oxoglutarate with concomitant reduction of NAD+ to NADH. The 2-oxoglutarate is recycled to l-glutamate in a transamination reaction catalyzed by d-PhgAT using d-4-hydroxyphenylglycine as an amino donor, which is converted to 4-hydroxybenzoylformate. Both NADH and 4-hydroxybenzoylformate strongly absorb UV light at 340 nm (ε340 nm=6.22×103 and 8.90×103 l mol−1 cm−1, respectively). The signal amplification effect of the cycling reactions is thus further enhanced by the combined absorption of the two accumulating reaction products. The standard calibration curve for l-glutamate was linear from 0.2 to 20 μM, with a detection limit of 0.14 μM. Food samples can be significantly diluted before subjected to the assay, thus reducing the effects of interfering substances. Because of the unique substrate specificity of d-PhgAT, l-glutamate could be selectively determined in the presence of other common amino acids at relatively high concentrations. The assay was satisfactorily applied to measure l-glutamate in various kinds of food products. The procedure is simple, rapid, accurate, and should be easily automated.
Keywords :
Enzymatic substrate cycling assay , Glutamate , Food , d-Phenylglycine aminotransferase
Journal title :
Analytica Chimica Acta
Journal title :
Analytica Chimica Acta