Title of article :
Validation of a high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of tetracyclines residues in bovine milk Original Research Article
Author/Authors :
Bernardete Ferraz Spisso، نويسنده , , Ana Luiza de Oliveira e Jesus، نويسنده , , Marcus Antônio Gonçalves de Ara?jo J?nior، نويسنده , , Mychelle Alves Monteiro، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
A high-performance liquid chromatography-fluorescence detection method was optimized and validated to determine tetracyclines residues in bovine milk. Post-column derivatization using metal complexation in non-aqueous reagent increased the fluorescence of chelates by a factor up to 2.54 compared to water (signal-to-noise ratio enhancement). Overall recoveries ranged from 61 to 115%, with RSDr from 5 to 15% (n = 54). Detection limits ranged from 5 to 35 μg kg−1. Limits of quantification were established at 50 μg kg−1. Decision limits (CCα) were 109, 108 and 124 μg kg−1 and detection capabilities (CCβ) 119, 117 and 161 μg kg−1 for oxytetracycline, tetracycline and chlortetracycline, respectively. The method was applied successfully in a national monitoring program.
Keywords :
Milk , Residues , Antibiotics , Tetracyclines , validation
Journal title :
Analytica Chimica Acta
Journal title :
Analytica Chimica Acta