Title of article :
Substrate 2′-Hydroxyl Groups Required for Ribozyme-Catalyzed Polymerization Original Research Article
Author/Authors :
Ulrich F. Müller، نويسنده , , David P. Bartel، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2003
Pages :
8
From page :
799
To page :
806
Abstract :
A polymerase ribozyme has been generated that uses nucleoside triphosphates to elongate an RNA primer by the successive addition of nucleotides complementary to an RNA template. Its polymerization is accurate, with an average error rate less than 3%, and it is general in terms of the sequence and the length of the primer and template RNAs. To begin to understand how the substrate contacts contribute to this accurate and general activity, we investigated which primer and template 2′-hydroxyl groups are involved in substrate recognition. We identified eight positions where 2′-deoxy substitutions can influence polymerization kinetics. All eight are within five nucleotides of the primer 3′ terminus. Some, but not all, of the 2′-deoxy effects appear to be sequence dependent. These results begin to build a picture of how the polymerase ribozyme recognizes its substrates.
Journal title :
Chemistry and Biology
Serial Year :
2003
Journal title :
Chemistry and Biology
Record number :
1158691
Link To Document :
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