Author/Authors :
Tateaki Wakamiya، نويسنده , , Ryusaku Togashi، نويسنده , , Takatoshi Nishida، نويسنده , , Kunio Saruta، نويسنده , , Jun-ichi Yasuoka، نويسنده , , Shoichi Kusumoto، نويسنده , , Saburo Aimoto and Yoshifumi Nishimura، نويسنده , , Kumiko Yoshizawa Kumagaye، نويسنده , , Kiichiro Nakajima، نويسنده , , Kazuhiro Nagata، نويسنده ,
Abstract :
Two kinds of phosphoserine-containing peptides related to HSP27 were synthesized by the Boc- or Fmoc-mode solid-phase method based on prephosphorylation strategy. In the case of the Boc strategy, the O-phosphono group of the phosphoserine residue was protected with the cyclopentyl or cyclohexyl group. On the other hand, N′-Fmoc-O-[(benzyloxy)hydroxyphosphinyl]serine was employed in case of the Fmoc strategy. Consequently, it has become feasible to utilize conventional solid-phase methods for synthesizing any phosphopeptides which are required to elucidate biochemical significance of protein phosphorylation.
