Title of article :
Toll-Like Receptors Expression in Follicular Cells of Patients with Poor Ovarian Response
Author/Authors :
Taghavi، Seyed Abdolvahab نويسنده Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , , Mehdizadeh، Mehdi نويسنده , , Karimian، Leili نويسنده Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , TAGHI JOGHATAIE، MOHAMMAD نويسنده , , Aflatoonian، Mohammad Reza نويسنده ,
Issue Information :
فصلنامه با شماره پیاپی 30 سال 2014
Abstract :
Background: Poor ovarian response (POR) to gonadotropin stimulation has led to
a significant decline in success rate of fertility treatment. The immune system may
play an important role in pathophysiology of POR by dysfunctions of cytokines and
the growth factor network, and the presence of ovarian auto-antibodies. The aim of
this study is to investigate the expression of toll-like receptors (TLR) 1, 2, 4, 5, 6 and
cyclooxygenase (COX) 2 genes in follicular cells and concentration of interleukin
(IL)-6, IL-8 and macrophage migration inhibitory factor (MIF), as major parts of
innate immunity, in follicular fluid (FF) obtained from POR women in comparison
with normal women.
Materials and Methods: In this case-control study, 20 infertile POR patients and 20
normal women took part in this study and underwent controlled ovarian stimulation.
The FF was obtained from the largest follicle ( > 18 mm). The FF was centrifuged
and cellular pellet was then used for evaluation of expression of TLRs and COX2
genes by real-time PCR. FF was used for quantitative analysis for IL-6, IL-8 and
MIF by enzyme-linked immunosorbent assay (ELISA).
Results: TLR1, 2, 4, 5, 6 and COX2 gene expression were significantly higher in POR
(p < 0.05). Concentration of IL-6, IL-8 and MIF proteins was significantly increased in
POR compared with normal women (p < 0.05).
Conclusion: These findings support the hypothesis that the immune system may be involved
in pathophysiology of POR through TLRs.
Journal title :
International Journal of Fertility and Sterility
Journal title :
International Journal of Fertility and Sterility