Title of article :
Growth characteristics of porcine chlamydial strains in different cell culture systems and comparison with ovine and avian chlamydial strains
Author/Authors :
Schiller، نويسنده , , Irene and Schifferli، نويسنده , , Andrea and Gysling، نويسنده , , Petra and Pospischil، نويسنده , , Andreas، نويسنده ,
Issue Information :
فصلنامه با شماره پیاپی سال 2004
Abstract :
Porcine Chlamydiaceae were cultivated under various culture conditions and we compared their growth characteristics with those of ruminant and avian strains.
mbination of centrifugation assisted cell culture infection and cycloheximide treatment of Vero cell coverslip cultures provided the highest inclusion numbers with all chlamydial strains. Interestingly, the use of Iscoveʹs modified Dulbeccoʹs medium instead of Eagleʹs minimal essential medium significantly increased Chlamydia suis inclusion counts. C. suis and Chlamydophila pecorum inclusion numbers were markedly increased in CaCo cells, compared with Vero cells. This accelerated growth of porcine Chlamydiaceae under certain cultivation conditions may be helpful for the propagation of low chlamydial numbers or for their isolation from field samples.
tracellular distribution of porcine Chlamydiaceae in polarised CaCo cells clearly demonstrated differences between the chlamydial strains: C. pecorum 1710S inclusions were predominantly localised in the apical cytoplasm, C. suis S45 inclusions, however, were mostly situated in lower cytoplasmatic compartments. These findings might reflect biological differences in vivo.
Keywords :
cell cultures , Polarised cells , Chlamydiaceae , Porcine
Journal title :
The Veterinary Journal
Journal title :
The Veterinary Journal