Title of article :
Selective and enhanced nitric oxide detection using hemoprotein/silica hybrids
Author/Authors :
Palaniappan، نويسنده , , Al. and Moochhala، نويسنده , , Shabbir and Tay، نويسنده , , Francis E.H. and Phua، نويسنده , , Nicky C.L. and Su، نويسنده , , Xiaodi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
3
From page :
241
To page :
243
Abstract :
We have attempted to detect nitric oxide (NO) in a gaseous state using soluble guanylyl cyclase (sGC), a NO specific hemoprotein, entrapped in a mesoporous silica network. Quartz crystal microbalance (QCM) carrying the above sensing matrix is used to monitor the NO response. The mesoporous silica film is prepared by a sol–gel process using polyethylene glycol with a molecular weight of 400 as an organic template to generate the porous structure. sGC is entrapped in the mesoporous silica network through physisorption. The frequency response of the QCM to NO is enhanced by the introduction of the mesoporous silica film on the QCM electrodes, and a detection sensitivity of ∼15 ppb/Hz is obtained. Owing to the strong affinity between the ferrous heme(s) of sGC and NO, we have demonstrated that other species such as nitrogen dioxide (NO2) and carbon monoxide (CO) exhibit minimal interferences on the sensor response and as such a selective NO detection is achieved.
Keywords :
hemoprotein , guanylyl cyclase , Biosensor , Nitric oxide , mesoporous silica , QCM
Journal title :
Sensors and Actuators B: Chemical
Serial Year :
2008
Journal title :
Sensors and Actuators B: Chemical
Record number :
1436318
Link To Document :
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