Author/Authors :
Hegnerov?، نويسنده , , K. and Bockov?، نويسنده , , M. and Vaisocherov?، نويسنده , , H. and Kri?tofikov?، نويسنده , , Z. and ???n?، نويسنده , , J. and ??pov?، نويسنده , , D. and Homola، نويسنده , , J.، نويسنده ,
Abstract :
This paper describes direct label-free detection of 17beta-hydroxysteroid dehydrogenase type 10 (17β-HSD10) using a surface plasmon resonance (SPR) biosensor. This multifunctional mitochondrial enzyme is involved in pathogenesis of Alzheimer disease (AD) and represents a potential target for AD diagnostics. A multichannel SPR sensor with spectral modulation is functionalized with amyloid beta or polyclonal antibody against 17β-HSD10 peptide using a self-assembled monolayer of alkylthiolates and amino coupling chemistry. Detection of a synthetic peptide of 14 amino acids from the 17β-HSD10 molecule (residues 133–146 aa) and whole enzyme 17β-HSD10 is performed. Detection of 17β-HSD10 enzyme in artificial cerebrospinal fluid (ACSF) buffer is also carried out. It is demonstrated that the reported SPR biosensor is capable of detecting 17β-HSD10 enzyme at ng/ml levels.
Keywords :
Alzheimer Disease , Amyloid beta , SPR sensor , 17?-HSD10