Title of article :
Screening method for inherited disorders of purine and pyrimidine metabolism by capillary electrophoresis with reversed electroosmotic flow
Author/Authors :
Adam، نويسنده , , Tom?? and Lochman، نويسنده , , Pavel and Friedeck?، نويسنده , , David، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Abstract :
Capillary electrophoresis with electroosmotic flow reversed by cationic surfactant for diagnosis of purine and pyrimidine inherited enzyme deficiencies is reported. Final separation conditions consist of 45 mM borate, 55 mM N-tris[hydroxymethyl]methylglycine, 10 mM tartrate, 1 mM cetyltrimethylammonium bromide and 0.44% tetrabutylammonium hydroxide-2-amino-2-methyl-1,3-propanediol (pH 8.6). Average sensitivity (2.51 μM), reproducibility of migration times (run-to-run C.V.≤0.6%, day-to-day C.V.≤2.5%), linearity (R2>0.994) and imprecision (mean intra-assay RSD 4.7% and inter-assay RSD 6.6%) of the method are acceptable for diagnostic purposes. Applicability of the method is demonstrated on urine samples from patients with enzymatically proven enzyme deficiences.
Keywords :
Purine , Pyrimidine
Journal title :
Journal of Chromatography B
Journal title :
Journal of Chromatography B