Title of article :
Isolation and identification of the glucuronide of 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methylamino)benzoic acid from rabbit urine
Author/Authors :
Dong، نويسنده , , Qingguang and Gu، نويسنده , , Jingkai and Zhong، نويسنده , , Dafang and Paul Fawcett، نويسنده , , J. and Chu، نويسنده , , Dafeng، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Pages :
4
From page :
369
To page :
372
Abstract :
The metabolic profile of 3H-1,2-dihydro-2-(4-methylphenylamino)methyl-1-pyrrolizinone (SFZ-47), a putative non-steroidal anti-inflammatory pro-drug, has been studied in rabbit urine. Semi-preparative reversed-phase HPLC of 24 h urine from two rabbits given single oral doses of SFZ-47 (200 mg) allowed the separation of SFZ-47 together with the oxidative metabolite 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methylamino)benzoic acid (SFZ-47-COOH) and its glucuronide conjugate. The glucuronide was characterized by ESI-MSn and 1H NMR and shown to be the 1-O-acyl β-d-glucuronide conjugate of SFZ-47-COOH. The method gave excellent resolution of the glucuronide from endogenous constituents in urine and may be suitable for the preparation of glucuronide metabolites of other drugs.
Keywords :
4-(3H-1 , 2-Dihydro-1-pyrrolizinone-2-methylamino)benzoic acid glucuronide
Journal title :
Journal of Chromatography B
Serial Year :
2002
Journal title :
Journal of Chromatography B
Record number :
1453671
Link To Document :
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