Title of article :
Simultaneous determination of JTT-501 and its main metabolite in human plasma by liquid chromatography–ionspray mass spectrometry
Author/Authors :
Breda، نويسنده , , M. and Basileo، نويسنده , , G. and James، نويسنده , , C.A.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Abstract :
An LC–MS–MS analytical method was developed for the determination of a new antidiabetic agent, JTT-501 and its main metabolite (JTP-20604) in human plasma. The compounds were isolated from plasma by protein precipitation before analysis by HPLC with atmospheric pressure positive ionisation MS–MS detection. An isotopically labelled analog of JTT-501 was used as the internal standard. Linearity was demonstrated over the calibration range of about 5–10 000 ng/ml for both compounds. The assay was validated with respect to accuracy, precision and analyte stability. This method was used for the determination of plasma concentrations for the two compounds in a clinical tolerability study. A cross-validation exercise between two different mass spectrometers, used for the determination of clinical samples, is also reported.
Keywords :
JTT-501 , JTP-20604
Journal title :
Journal of Chromatography B
Journal title :
Journal of Chromatography B