Title of article :
Determination of disodium clodronate in human plasma and urine using gas-chromatography–nitrogen-phosphorous detections: validation and application in pharmacokinetic study
Author/Authors :
Muntoni، نويسنده , , E. and Canaparo، نويسنده , , R. and Della Pepa، نويسنده , , C. and Serpe، نويسنده , , L. and Casale، نويسنده , , F. and Barbera، نويسنده , , S. and Romano، نويسنده , , P. and Zara، نويسنده , , G.P. and Eandi، نويسنده , , M.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Abstract :
We present a specific method for the determination of disodium clodronate in human plasma and urine using a gas-chromatographic system with nitrogen phosphorus detector (NPD). The compound was extracted from plasma and urine samples by an anion-exchange resin and derivatizated with bistrimethylsilyltrifluoroacetamide (BSTFA). Sodium bromobisphosphonate was used as internal standard. The calibration curves were linear in both plasma and urine, with a regression coefficient r>0.9975 in plasma and r>0.9977 in urine.
mit of quantitation was 0.3 μg/ml in plasma and 0.5 μg/ml in urine. The method was validated by intra-day assays at three concentration levels. During the study we carried out inter-day assays to confirm the feasibility of the method. The precision in plasma at 0.5, 15, and 45 μg/ml was 12.4, 0.2, and 6.5% (n=40), respectively; in urine at 0.8, 8, and 40 μg/ml it was 8.6, 6.4, and 9.3% (n=40), respectively.
thod was accurate and reproducible, and was successfully applied to determine the pharmacokinetic parameters of clodronate in healthy volunteers after intravenous infusion and intramuscular injection of 200 mg of the compound. The Cmax after intravenous infusion and intramuscular injection was 16.1 and 12.8 μg/ml, respectively. AUC0–48 h after infusion administration and intramuscular injection was 44.2±18.0 and 47.5±12.4 h μg/ml, respectively. The elimination half-life in both administrations was 6.31±2.7 h.
Keywords :
Pharmacokinetics , Disodium clodronate , Validation
Journal title :
Journal of Chromatography B
Journal title :
Journal of Chromatography B