Title of article :
Quantitative analysis of Tenecteplase in rat plasma samples using LC–MS/MS as an alternative for ELISA
Author/Authors :
Buscher، نويسنده , , B.A.P. and Gerritsen، نويسنده , , H. and van Schِll، نويسنده , , I. and Cnubben، نويسنده , , N.H.P. and Brüll، نويسنده , , L.P.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
An LC–MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; MW 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats.
Keywords :
Proteins , PLASMA , digestion , Liquid chromatography , Quantitative analysis , mass spectrometry
Journal title :
Journal of Chromatography B
Journal title :
Journal of Chromatography B