Author/Authors :
Xue، نويسنده , , Yanfeng and Wei، نويسنده , , Junying and Han، نويسنده , , Huanhuan and Zhao، نويسنده , , Liyan and Cao، نويسنده , , Dong and Wang، نويسنده , , Jinglan and Yang، نويسنده , , Xiaoming J. Zhang، نويسنده , , Yangjun and Qian، نويسنده , , Xiaohong، نويسنده ,
Abstract :
A new approach utilizing open tubular capillary columns coated with zirconium phosphonate (ZrP-OTCC) for enrichment of phosphopeptides is described. The experimental conditions: interior diameter, length of capillary and flow rate was optimized using tryptic digest of α-casein (a phosphoprotein) as a model sample. The ZrP-OTCC was demonstrated to tolerate urea, sodium dodecyl sulphate (SDS), and NaCl. Further experimental results show that the ZrP-OTCC can trap the phosphopeptides even at the concentration of α-casein as low as 10−8 M. This column has also been successfully coupled online with nano-liquid chromatography for enrichment and then separation of phosphopeptides from a complex sample, and finally analyzed the phosphopeptides by mass spectrometry (MS).
Keywords :
Phosphopeptides , Open tubular capillary columns , mass spectrometry , Zirconium phosphonate
