Title of article :
A sensitive and selective liquid chromatography/tandem mass spectrometry method for determination of MLN8237 in human plasma
Author/Authors :
Lipsitz، نويسنده , , Emily and Moorthy، نويسنده , , Ganesh and Mosse، نويسنده , , Yael and Fox، نويسنده , , Elizabeth and Adamson، نويسنده , , Peter C.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
5
From page :
2369
To page :
2373
Abstract :
We describe a selective and a highly sensitive high-performance liquid chromatography–electron spray ionization-collision induced dissociation-tandem mass spectrometry (HPLC–ESI-CID-MS/MS) assay for the Aurora A kinase inhibitor MLN8237 in human plasma. The intra-day precision based on the standard deviation of replicates of quality control samples ranged from 0.2 to 4% and with accuracy ranging from 96 to 102%. The inter-day precision ranged from 0.5 to 7% and the accuracy ranged from 93 to 105%. Stability studies showed that MLN8237 was stable both during the expected conditions for sample preparation and storage. The lower limit of quantification for MLN8237 was 5 ng/mL. The analytical method showed excellent sensitivity, precision, and accuracy. This method is robust and is being successfully employed in a Childrenʹs Oncology Group Phase 1 Consortium study of MLN8237 in children with cancer.
Keywords :
MLN8237 , PLASMA , CANCER , LC–ESI-CID-MS/MS , Pediatric
Journal title :
Journal of Chromatography B
Serial Year :
2010
Journal title :
Journal of Chromatography B
Record number :
1468745
Link To Document :
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