Abstract :
A quick and direct method for measuring tocopherols (α, β+γ and δ) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (α-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol–water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).
