Title of article :
Towards functional group-specific detection in high-performance liquid chromatography using mid-infrared quantum cascade lasers
Author/Authors :
Edelmann، نويسنده , , Andrea and Ruzicka، نويسنده , , Caterina and Frank، نويسنده , , Johannes and Lendl، نويسنده , , Bernhard and Schrenk، نويسنده , , Werner and Gornik، نويسنده , , Erich and Strasser، نويسنده , , Gottfried، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Pages :
6
From page :
123
To page :
128
Abstract :
A distributed feedback quantum cascade laser was applied for the first time as a powerful light source for mid-infrared (MIR) detection in liquid chromatography. Fructose and glucose in red wine were separated with an isocratic HPLC system, which was connected to a custom-made flow cell. This flow cell was constructed of two diamond windows with adjustable spacing and two hollow wave-guides for guiding the incoming and outgoing light. The HPLC column based on an ion-exchange resin with calcium(II) counter ion was run at 80°C with 0.04% formic acid as the mobile phase. Under these conditions the carbohydrates could not be completely separated from the organic acids also present in wine. However, the emission of the laser at 1067 cm−1 matches the absorption maximum of fructose and glucose, whereas the organic acids do not absorb appreciably at this wavenumber. Thus group-specific detection could be achieved. Additionally, the optical path length could be increased from 25 to 125 μm, which is very promising in gaining enhanced sensitivity compared to Fourier transform IR detection.
Keywords :
carbohydrates
Journal title :
Journal of Chromatography A
Serial Year :
2001
Journal title :
Journal of Chromatography A
Record number :
1508516
Link To Document :
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