Title of article :
Simultaneous determination of vinclozolin and detection of its degradation products in mouse plasma, serum and urine, and from rabbit bile, by high-performance liquid chromatography
Author/Authors :
Dhananjeyan، نويسنده , , Mugunthu R. and Erhardt، نويسنده , , Paul W. and Corbitt، نويسنده , , Cynthia، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Abstract :
A specific high-performance liquid chromatography method has been developed for simultaneous detection of vinclozolin and its degradation products (M1, M2, and M3). The method has been validated according to ICH guidelines and can be extended to quantitation of vinclozolin. A base-line separation of vinclozolin and its degradation products was found with symmetrical peak shapes on an XTerra™ MS C18 column using 10 mM ammonium bicarbonate at pH 9.2 and acetonitrile as mobile phase. The retention times of vinclozolin, M1, M2, and M3 were 12.8, 8.1, 11.6, and 11.1 min, respectively. A linear calibration curve was obtained across a range from 5 to 200 μM for vinclozolin. The intra- and inter-day relative standard deviations (%RSD) were <1%. Greater than 90% recoveries of vinclozolin from bio-fluids including mouse plasma, serum and urine, and rabbit bile, were obtained in a single step with a single solvent.
Keywords :
anti-androgens , endocrine disruptors , Vinclozolin
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A