Title of article :
Modeling species-specific diacylglycerol dynamics in the RAW 264.7 macrophage
Author/Authors :
Callender، نويسنده , , Hannah L. and Horn، نويسنده , , Mary Ann and DeCamp، نويسنده , , Dianne L. and Sternweis، نويسنده , , Paul C. and Alex Brown، نويسنده , , H.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
12
From page :
679
To page :
690
Abstract :
A mathematical model of the G protein signaling pathway in RAW 264.7 macrophages downstream of P 2 Y 6 receptors activated by the ubiquitous signaling nucleotide uridine 5 ’-diphosphate is developed. The model, which is based on time-course measurements of inositol trisphosphate, cytosolic calcium, and diacylglycerol, focuses particularly on differential dynamics of multiple chemical species of diacylglycerol. When using the canonical pathway representation, the model predicted that key interactions were missing from the current network structure. Indeed, the model suggested that accurate depiction of experimental observations required an additional branch to the signaling pathway. An intracellular pool of diacylglycerol is immediately phosphorylated upon stimulation of an extracellular receptor for uridine 5 ’-diphosphate and subsequently used to aid replenishment of phosphatidylinositol. As a result of sensitivity analysis of the model parameters, key predictions can be made regarding which of these parameters are the most sensitive to perturbations and are therefore most responsible for output uncertainty.
Keywords :
Sensitivity analysis , Cellular signaling pathways , Mathematical Modeling , P 2 Y 6 receptor , Uridine 5 ’-diphosphate
Journal title :
Journal of Theoretical Biology
Serial Year :
2010
Journal title :
Journal of Theoretical Biology
Record number :
1540011
Link To Document :
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