Title of article :
Influence of flavan-3-ols and procyanidins on UVC-mediated formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine in isolated DNA
Author/Authors :
Ottaviani، نويسنده , , Javier I and Carrasquedo، نويسنده , , Fernando and Keen، نويسنده , , Carl L and Lazarus، نويسنده , , Sheryl A and Schmitz، نويسنده , , Harold H and G. Fraga، نويسنده , , Cesar، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Pages :
6
From page :
203
To page :
208
Abstract :
The flavan-3-ols (−)-epicatechin (epicatechin) and (+)-catechin (catechin) and their related oligomers (procyanidins) isolated from cocoa were assayed for their capacity to inhibit the UVC-mediated formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (oxo8dG) in calf thymus DNA. The above-mentioned compounds inhibited oxo8dG production in a concentration- and time-dependent manner. After 30 min of irradiation (30 kJ/m2), 0.1, 1.0, 10, and 100 μM epicatechin inhibited oxo8dG formation by 20, 36, 64, and 74%, respectively. For the same dose of UVC, 0.1, 1.0, 10, and 100 μM catechin inhibited oxo8dG formation by 1, 23, 50, and 70%, respectively. Epicatechin was more efficient than catechin with respect to inhibiting oxo8dG formation (IC50 1.7 ± 0.7 vs 4.0 ± 0.7 μM). Monomer, tetramer, and hexamer fractions were equally effective in inhibiting oxo8dG formation when assayed at 10 μM monomer equivalent concentration. At similar concentrations (1–50 μM), the inhibition of the UVC-mediated oxo8dG formation by flavan-3-ols and procyanidins was in the range of that of α-tocopherol, Trolox, ascorbate, and glutathione. These results support the concept that flavan-3-ols and their related procyanidins can protect DNA from oxidation at concentrations that can be physiologically relevant. Both epimerism and degree of oligomerization are important determinants of the antioxidant activity of flavan-3-ols and procyanidins.
Keywords :
Cocoa , Cardiovascular disease , Mutation , Flavan-3-ols , Flavonoids , Polyphenols , antioxidants
Journal title :
Archives of Biochemistry and Biophysics
Serial Year :
2002
Journal title :
Archives of Biochemistry and Biophysics
Record number :
1619891
Link To Document :
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