Abstract :
A new method is presented for the determination of DMA in water as its 9-fluorenylmethyl chloroformate (FMOC) derivative using solid-phase microextraction (SPME) and liquid chromatography. The method is based on the employment of SPME fibres coated with carbowax-templated resin (CW-TR) for analyte extraction and derivatization. The fibres were successively immersed in the samples, in a solution of o-phthalaldialdehyde and N-acethyl-l-cysteine (OPA–NAC) and finally, in a solution of FMOC. OPA–NAC reacted on the fibre with possible primary aliphatic amines present in the samples, particularly with PA which is a direct interferent in the determination of DMA with FMOC. In such a way, the formation of PA–FMOC during the second stage was prevented, and thus the method was selective for DMA. The proposed procedure was applied to the determination of DMA in the 1.0–10.0 μg/mL range. The method provided suitable linearity, accuracy and reproducibility, and limits of detection and quantification of 0.3 and 1.0 μg/mL, respectively. The applicability of the method for the determination of DMA in different types of water is shown.
