Title of article :
In situ electrodeposited nanoparticles for facilitating electron transfer across self-assembled monolayers in biosensor design
Author/Authors :
Du، نويسنده , , Dan and Ding، نويسنده , , Jiawang and Cai، نويسنده , , Jie and Zhang، نويسنده , , Jianming and Liu، نويسنده , , Li، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2008
Pages :
7
From page :
1337
To page :
1343
Abstract :
Gold nanoparticles (AuNPs) were synthesized in situ and electrodeposited onto Au substrate. The AuNPs modified interface facilitates electron transfer across self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA). After activation of surface carboxyl groups with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, the interface displayed good stability for immobilization of biomolecules. These modification processes were characterized by contact angle measurement, cyclic voltammetry and electrochemical impedance spectra. The immobilized acetylcholinesterase (AChE), as a model, showed excellent activity to its substrate, leading to a stable AChE biosensor. Under the optimal experimental conditions, the inhibition of malathion on AChE biosensor was proportional to its concentration in two ranges, from 0.001 to 0.1 μg mL−1 and from 0.1 to 25 μg mL−1, with detection limit of 0.001 μg mL−1. The simple method showed good reproducibility and acceptable stability, which had potential application in biosensor design.
Keywords :
Biosensor , Gold nanoparticles , Electrodeposition , Self-assembled monolayers , organophosphate pesticides
Journal title :
Talanta
Serial Year :
2008
Journal title :
Talanta
Record number :
1654115
Link To Document :
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