Title of article :
Hydrazide-functionalized magnetic microspheres for the selective enrichment of digested tryptophan-containing peptides in serum
Author/Authors :
Yu، نويسنده , , Yanyan and Liu، نويسنده , , Mingqi and Yan، نويسنده , , Guoquan and He، نويسنده , , Yifeng and Xu، نويسنده , , Congjian and Shen، نويسنده , , Huali and Yang، نويسنده , , Pengyuan، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2011
Abstract :
The extreme complexity of protein samples is becoming a great challenge for proteomic analysis, especially for those having large dynamic range of protein abundance. To solve this problem, and to overcome the limitation of the current proteomic technologies, a new method using hydrazide-functionalized magnetic microspheres was established in this study. With this method, tryptophan (Trp)-containing peptides can be selectively and sensitively enriched from complex and low-volume samples. Furthermore, combined with 1D-LC–MS/MS analysis, the strategy was successfully applied to the proteomic study of mouse serum. The proportion of Trp-containing peptides was increased from 19.4% to 80.2% through enrichment, and the complexity of the sample was reduced more than two times. An additional 113 Trp-containing peptides and 48 novel proteins were detected compared to the conventional method. This enrichment method provides a means for identifying more proteins as potential biomarkers in serum and other complex samples.
Keywords :
Tryptophan-containing peptides , Selective enrichment , mass spectrometry , serum , Hydrazide-functionalized magnetic microspheres