Title of article :
Simultaneous determination of flavonoid analogs in Scutellariae Barbatae Herba by β-cyclodextrin and acetonitrile modified capillary zone electrophoresis
Author/Authors :
Li، نويسنده , , Yuan-Yuan and Zhang، نويسنده , , Qingfeng and Sun، نويسنده , , Hongyan and Cheung، نويسنده , , Nga-Ki and Cheung، نويسنده , , Hon-Yeung and Zhang، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2013
Pages :
10
From page :
393
To page :
402
Abstract :
A capillary zone electrophoresis (CZE) method modified by β-cyclodextrin (β-CD) and acetonitrile (ACN) was developed for simultaneous determination of seven structurally similar flavonoids in Scutellariae Barbatae Herba (SBH) and its preparations. Molecular selectivity of the analytes by β-CD was in the following order: apigenin, luteolin, quercetin, scutellarin, baicalein, rutin and wogonin, based mainly on the “molecular fit” interaction between some ligands in the C ring of the flavonoid and the cavity of β-CD. Flavonoids with hydroxyl substituent(s) at the C-ring, especially the 4′ monohydroxyl, were highly selected by β-CD although hydrophobicity of the guest molecule is the primary factor affecting the complexation. The function of acetonitrile in this study was to improve the separation of the analytes in the real SBH. The developed method was validated and applied to real samples. The principle of separation based on this CZE condition is also explained.
Keywords :
Capillary zone electrophoresis , Flavonoids , Separation behavior , Scutellariae Barbatae Herba , Molecular selectivity , ?-Cyclodextrin
Journal title :
Talanta
Serial Year :
2013
Journal title :
Talanta
Record number :
1667005
Link To Document :
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