Author/Authors :
Jin Choi، نويسنده , , Sung and Lee، نويسنده , , Hee-Yong and Beam Kim، نويسنده , , Sang and Kim، نويسنده , , Jeong-Han and Seok Lee، نويسنده , , Seung and Dong Yoo، نويسنده , , Sun and Choon Lee، نويسنده , , Kang and Suk Lee، نويسنده , , Hye، نويسنده ,
Abstract :
A novel HPLC method with electrochemical detection is described for the determination of a growth-hormone-releasing hexapeptide (GHRP-6). HPLC conditions, such as the column, mobile phase, and oxidation potential, were optimized for sensitivity and selectivity of analysis. GHRP-6 was separated on a reversed-phase CN column with 37% acetonitrile in 100 mM phosphate buffer (pH 7.0) as the mobile phase. The optimum electrochemical oxidation signal was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode due to two electroactive tryptophans and a histidine residue. Solid-phase extraction using octadecyl cartridges was optimized for sample cleanup of GHRP-6 from serum samples and the method was successfully applied over the concentration range of 5 to 100 ng/ml of analyte.
