Title of article :
Injectable and porous PLGA microspheres that form highly porous scaffolds at body temperature
Author/Authors :
L.J. and Qutachi، نويسنده , , Omar and Vetsch، نويسنده , , Jolanda R. and Gill، نويسنده , , Daniel and Cox، نويسنده , , Helen and Scurr، نويسنده , , David J. and Hofmann، نويسنده , , Sandra and Müller، نويسنده , , Ralph and Quirk، نويسنده , , Robin A. and Shakesheff، نويسنده , , Kevin M. and Rahman، نويسنده , , Cheryl V.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
9
From page :
5090
To page :
5098
Abstract :
Injectable scaffolds are of interest in the field of regenerative medicine because of their minimally invasive mode of delivery. For tissue repair applications, it is essential that such scaffolds have the mechanical properties, porosity and pore diameter to support the formation of new tissue. In the current study, porous poly(dl-lactic acid-co-glycolic acid) (PLGA) microspheres were fabricated with an average size of 84 ± 24 μm for use as injectable cell carriers. Treatment with ethanolic sodium hydroxide for 2 min was observed to increase surface porosity without causing the microsphere structure to disintegrate. This surface treatment also enabled the microspheres to fuse together at 37 °C to form scaffold structures. The average compressive strength of the scaffolds after 24 h at 37 °C was 0.9 ± 0.1 MPa, and the average Young’s modulus was 9.4 ± 1.2 MPa. Scaffold porosity levels were 81.6% on average, with a mean pore diameter of 54 ± 38 μm. This study demonstrates a method for fabricating porous PLGA microspheres that form solid porous scaffolds at body temperature, creating an injectable system capable of supporting NIH-3T3 cell attachment and proliferation in vitro.
Keywords :
PLGA , Microsphere , Scaffold , porosity , Cell delivery
Journal title :
Acta Biomaterialia
Serial Year :
2014
Journal title :
Acta Biomaterialia
Record number :
1758606
Link To Document :
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