Title of article :
Isolation and characterization of human breast cancer cells overexpressing S-adenosylmethionine decarboxylase
Author/Authors :
Manni، نويسنده , , Andrea and Badger، نويسنده , , Betty and Grove، نويسنده , , Rhea and Kunselman، نويسنده , , Susan and Demers، نويسنده , , Laurence، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1995
Pages :
6
From page :
23
To page :
28
Abstract :
We report the first successful isolation and initial characterization of S-adenosylmethionine decarboxylase (SAMDC)-overexpressing cells using a transfection approach. Stably transfected MCF-7 breast cancer cells overproducing SAMDC (~5-fold) manifested reduced ornithine decarboxylase while levels of N′-spermidine/spermine acetyltransferase were variably increased. Analysis of cellular polyamine profile showed that spermine was selectively increased (~80%), while spermidine and putrescine levels were reduced (~48% and ~15% of control, respectively). Since SAMDC-overexpressing clones exhibited increased clonogenicity in soft agar, our data suggest that spermine may be selectively involved in conferring a more invasive phenotype to breast cancer cells.
Keywords :
S-adenosylmethionine decarboxylase , Soft agar clonogenicity , Proliferative activity , Human breast cancer cells , polyamines
Journal title :
Cancer Letters
Serial Year :
1995
Journal title :
Cancer Letters
Record number :
1814871
Link To Document :
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