Title of article :
Dysregulation of overexpressed IL-32α in hepatocellular carcinoma suppresses cell growth and induces apoptosis through inactivation of NF-κB and Bcl-2
Author/Authors :
Kang، نويسنده , , Yun Hee and Park، نويسنده , , Mi-Young and Yoon، نويسنده , , Do-Young and Han، نويسنده , , Seung Ro and Lee، نويسنده , , Chung Il and Ji، نويسنده , , Na Young and Myung، نويسنده , , Pyung-Keun and Lee، نويسنده , , Hee Gu and Kim، نويسنده , , Jae Wha and Yeom، نويسنده , , Young Il and Jang، نويسنده , , Ye Jin and Ahn، نويسنده , , Dong Kuk and Kim، نويسنده , , Jong Wan and Song، نويسنده , , Eun Young، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
8
From page :
226
To page :
233
Abstract :
IL-32 is a newly discovered cytokine. Recently, various reports suggest that it plays a role as a proinflammatory mediator and may be involved in several cancer carcinogenesis. However, IL-32 expression in hepatocellular carcinoma (HCC) remains unclear. In this study, we investigated the expression and role of IL-32α in hepatocellular carcinoma, because IL-32 was identified as an upregulated gene in hepatocellular carcinoma tissues compared to nontumorous regions using DNA microarray. IL-32α was overexpressed in tissue and serum from patients with HCC and localized in the cytoplasm and nucleus of hepatocellular carcinoma tumor cells. Moreover, secreted IL-32α concentration in the serum of patients with hepatocellular carcinoma was elevated as compared with those in the normal serum using a developed sandwich ELISA. Furthermore, IL-32α suppression in hepatocellular carcinoma decreased expression of phospho-p38 MAPK, NF-κB, and antiapoptotic protein Bcl-2 and induced expression of proapoptotic proteins as well as p53 and PUMA resulting in the suppression of cell growth and induction of intrinsic apoptosis. Based on our results, we suggest that IL-32α is involved in the progression of hepatocellular carcinoma and may be a useful biomarker for diagnosis and therapeutic target of hepatocellular carcinoma.
Keywords :
IL-32? , hepatocellular carcinoma , Diagnostic marker , apoptosis , cell growth
Journal title :
Cancer Letters
Serial Year :
2012
Journal title :
Cancer Letters
Record number :
1821266
Link To Document :
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