Author/Authors :
Mostert، نويسنده , , Bianca and Kraan، نويسنده , , Jaco and Sieuwerts، نويسنده , , Anieta M. and van der Spoel، نويسنده , , Petra and Bolt-de Vries، نويسنده , , Joan and Prager-van der Smissen، نويسنده , , Wendy J.C. and Smid، نويسنده , , Marcel and Timmermans، نويسنده , , Annemieke M. and Martens، نويسنده , , John W.M. and Gratama، نويسنده , , Jan W. and Foekens، نويسنده , , John A. and Sleijfer، نويسنده , , Stefan، نويسنده ,
Abstract :
Circulating tumor cells (CTCs) can be enumerated using CellSearch, but not all breast cancer subtypes, specifically those with epithelial-mesenchymal transition (EMT) characteristics, sufficiently express the enrichment (EpCAM) and selection (CK8/18/19) markers used in this method. While CD146 can detect EpCAM-negative CTCs, we here evaluated the value of various cytokeratins and CD49f to detect CK8/18/19-negative CTCs. The tested cytokeratins provided no substantial benefit, but adding CD49f to CK8/18/19 as a selection marker resulted in improved recovery of normal-like cell lines.
ed staining of CK8/18/19 and CD49f after CD146/EpCAM enrichment is likely to further improve CTC detection in breast cancer.
Keywords :
Circulating tumor cells , Cytokeratin , CellSearch , breast cancer , CD49f
