Author/Authors :
Zhao، نويسنده , , Shuai and Ohara، نويسنده , , Shiori and Kanno، نويسنده , , Yuichiro and Midorikawa، نويسنده , , Yudai and Nakayama، نويسنده , , Momoka and Makimura، نويسنده , , Minami and Park، نويسنده , , Youngjin and Inouye، نويسنده , , Yoshio، نويسنده ,
Abstract :
The interaction between HER2 and aryl hydrocarbon receptor (AhR) signaling cascades during mammosphere formation of MCF-7 cells was studied. A newly established clonal MCF-7 cell line (HER2-5), stably overexpressing HER2, showed significantly enhanced levels of AhR mRNA and protein compared with MCF-7 cells. AhR was required for the HER2-mediated induction of interleukin-6 mRNA and for mammosphere formation in HER2-5 and MCF-7 cells. Mammosphere forming efficiency was suppressed by an AhR antagonist in a dose-dependent manner, as well as by knockdown of AhR. Taken together, these results indicate that AhR enhances mammosphere formation by human HER2-overexpressing breast cancer cells.
Keywords :
Aryl Hydrocarbon Receptor , IL-6 , MCF-7 , HER2 , Cancer stem cells
