Author/Authors :
Komura، نويسنده , , Dirce L. and Carbonero، نويسنده , , Elaine R. and Gracher، نويسنده , , Ana Helena P. and Baggio، نويسنده , , Cristiane H. and Freitas، نويسنده , , Cristina S. and Marcon، نويسنده , , Rodrigo and Santos، نويسنده , , Adair R.S. and Gorin، نويسنده , , Philip A.J. and Iacomini، نويسنده , , Marcello، نويسنده ,
Abstract :
Fucogalactans from Agaricus brasiliensis (EPF-Ab) and A. bisporus var. hortensis (EPF-Ah) were prepared via by aqueous extraction and a purification procedure. EPF-Ab had Mw 19.4 × 103 g/mol and EPF-Ah Mw 31.1 × 103 g/mol. EPF-Ab had a (1 → 6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp. EPF-Ah had a similar main-chain with O-2 substitution, but was partially methylated at HO-3, as well as having 2.5% non-reducing end-units of β-D-Gal. In mice, EPF-Ab gave 39% antinociceptive inhibition (ID50 > 100 mg/kg) and no anti-inflammatory activity. EPF-Ah also gave an inhibition of 39% at ID50 0.33 mg/kg and also inhibited by 61% (ID50 5.0 mg/kg) total cell migration and by 32% peritoneal capillary permeability, which is related to the anti-inflammatory effect. The small differences in chemical structure in these polysaccharides thus modified their biological activities.
Keywords :
Agaricus spp. , Fucogalactans , NMR spectroscopy , Antinociceptive , Anti-inflamatory
