Title of article :
Monitoring bacterial community shifts in bioleaching of Ni–Cu sulfide
Author/Authors :
He، نويسنده , , Zhiguo and Zhao، نويسنده , , Jiancun and Gao، نويسنده , , Fengling and Hu، نويسنده , , Yuehua and Qiu، نويسنده , , Guanzhou، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
7
From page :
8287
To page :
8293
Abstract :
The microbial ecology of the bioleaching of Ni–Cu sulfide is poorly understood and little effort has been made to handle the microbiological components of these processes. In this study, denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes fragments from bacteria was used to evaluate the changes of the bacterial community in the process of Ni–Cu sulfide bioleaching in a shaken flask system. The results revealed that the bacterial community was disturbed after the addition of Ni–Cu sulfide. Phylogenetic analyses of 16S rRNA fragments revealed that the retrieved sequences clustered together with the genera Acidithiobacillus and Leptospirillum. Multidimensional scaling (MDS) and cluster analysis of DGGE-banding patterns revealed that the process of Ni–Cu sulfide bioleaching in 46 days was divided into three stages. During the bioleaching process of Ni–Cu sulfide, Leptospirillum was always dominant. The genera Acidithiobacillus was only detected at early and later stages of the bioleaching process. These results extend our knowledge on microbial dynamics in Ni–Cu sulfide bioleaching, a key issue required to improve commercial applications.
Keywords :
DGGE , Bioleaching , RFLP , Acid mine drainage , Ni–Cu sulfide
Journal title :
Bioresource Technology
Serial Year :
2010
Journal title :
Bioresource Technology
Record number :
1922027
Link To Document :
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