Title of article :
Sugarcane bagasse degradation and characterization of three white-rot fungi
Author/Authors :
Dong، نويسنده , , Xiu Qin and Yang، نويسنده , , Jin Shui and Zhu، نويسنده , , Ning and Wang، نويسنده , , En Tao and Yuan، نويسنده , , Hong Li، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Abstract :
In order to investigate the details of lignin biodegradation, the characteristics and process of sugarcane bagasse (SCB) degradation by three lignin degrading fungi, Phanerochaete chrysosporium PC2, Lentinula edode LE16 and Pleurotus ostreatus PO45, were studied. We found that the ligninolytic enzymes polyphenol oxidase (PPO) and manganese peroxidase (MnP) were produced first, and that the cellulolytic enzyme CMCase was produced subsequently. These three fungi were more efficient to degrade lignin (85–93%) than hemicelluloses (64–88%) and cellulose (15–67%) in 12 weeks, in which P. chrysosporium PC2 was the most efficient strain to degrade all the ingredients. Results of the FTIR and CP/MAS 13C NMR revealed that the three fungi preferentially degraded syringyl units. The PPO and MnP as the main ligninolytic enzymes, especially the presence of PPO, were new findings in this study, which improved our knowledge of biopretreatment of SCB and evidenced these strains as valuable resource for SCB biotransformation.
Keywords :
sugarcane bagasse , lignin , Manganese peroxidase , White-rot fungi , Polyphenol oxidase
Journal title :
Bioresource Technology
Journal title :
Bioresource Technology