Author/Authors :
Dong، نويسنده , , Yichun and Xia، نويسنده , , Xi and Wang، نويسنده , , Xia and Ding، نويسنده , , Shuangyang and Li، نويسنده , , Xiaowei and Zhang، نويسنده , , Suxia and Jiang، نويسنده , , Haiyang and Liu، نويسنده , , Jinfeng and Li، نويسنده , , Jiancheng and Feng، نويسنده , , Zhongze and Ye، نويسنده , , Ni and Zhou، نويسنده , , Mingxia and Shen، نويسنده , , Jianzhong، نويسنده ,
Abstract :
A sensitive and reliable method was developed and validated for the analysis of ractopamine in swine tissues. The sample preparation procedure was based on hydrolysis overnight, extraction with ethyl acetate, and solid-phase extraction cleanup. The target compound was determined by ultra-performance liquid chromatography coupled with tandem mass spectrometry. The average recoveries ranged between 93.3% and 106.5%, with relative standard deviations of 7.2–12.8%. The detection and quantification limits were 0.2 and 0.5 μg/kg, respectively. The depletion profile of ractopamine was studied in healthy pigs after oral administration of feed containing 20 mg/kg ractopamine for 30 consecutive days. Ractopamine residues were still detected 11 days post-medication in all tissues examined with the exception of muscle. The highest ractopamine level was found in lung tissue. The developed method has been successfully applied to the depletion study of ractopamine in swine tissues.
Keywords :
Tandem mass spectrometry , Swine , Residue depletion , Ractopamine , Ultra-performance liquid chromatography
