Title of article :
Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface
Author/Authors :
Wang، نويسنده , , Peng and Li، نويسنده , , Hai and Yang، نويسنده , , Hai Jun and Wang، نويسنده , , Hua Bing and Lü، نويسنده , , Jun Hong and Zhang، نويسنده , , Yi and Hu، نويسنده , , Jun، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Pages :
4
From page :
264
To page :
267
Abstract :
Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed.
Keywords :
dynamic light scattering , Atomic Force Microscope , Disaggregation , Glycerol , Recombinant adeno-associated virus 2 (rAAV2)
Journal title :
Colloids and Surfaces B Biointerfaces
Serial Year :
2007
Journal title :
Colloids and Surfaces B Biointerfaces
Record number :
1968589
Link To Document :
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