Title of article :
Solid phase extraction using magnetic core mesoporous shell microspheres with C18-modified interior pore-walls for residue analysis of cephalosporins in milk by LC–MS/MS
Author/Authors :
Liu، نويسنده , , Xiaodan and Yu، نويسنده , , Yingjia and Zhao، نويسنده , , Meiyan and Zhang، نويسنده , , Haiying and Li، نويسنده , , Yan and Duan، نويسنده , , Gengli، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
7
From page :
206
To page :
212
Abstract :
A fast and effective extraction method has been developed for measuring the residue of cephalosporins (cefalexin, cefazolin, cefoperazone) in milk by using magnetic core-mesoporous shell microspheres with C18-functionalized interior pore-walls (C18-Fe3O4@mSiO2) as adsorbent. With no need for any protein precipitation procedure, the cephalosporins were directly adsorbed onto the C18-Fe3O4@mSiO2 microspheres through hydrophobic interaction with C18-groups (Octadecyl functional groups) functionalized in the interior walls of mesopore channels while the abundant proteins in milk sample were excluded out of the channel due to the size exclusion effect. Thereafter, the cephalosporins-absorbed C18-Fe3O4@mSiO2 microspheres were rapidly isolated by placing a magnet, and followed by liquid chromatography–tandem mass spectrometry analysis after eluted by methanol. Various parameters which could affect the extraction performance were optimised. The newly developed extraction method was successfully applied in determination of cephalosporin residues in milk samples, offering a valuable alternative to simplify and speed up the sample preparation step.
Keywords :
C18-modified interior pore-walls , extraction , Magnetic mesoporous microspheres , Cephalosporins , Milk samples , LC–MS/MS
Journal title :
Food Chemistry
Serial Year :
2014
Journal title :
Food Chemistry
Record number :
1976468
Link To Document :
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