Author/Authors :
Zhang، نويسنده , , Juan and Li، نويسنده , , Ran and Jiang، نويسنده , , Feng-Lei and Zhou، نويسنده , , Bo and Luo، نويسنده , , Qing-Ying and Yu، نويسنده , , Qiu-Li-Yang and Han، نويسنده , , Xiao-Le and Lin، نويسنده , , Yi and He، نويسنده , , Hong and Liu، نويسنده , , Yi and Wang، نويسنده , , Yun-Long، نويسنده ,
Abstract :
In this work, we proposed an immobilization scheme targeting Escherichia coli bacterial cells onto gold surface utilizing polypeptide RGDʹs binding specificity to gold particles. Adsorption kinetics of extracellular Herring Sperm DNA on E. coli was then studied using electrochemical methods along with surface plasmon resonance spectroscopy through this immobilization scheme. The adsorption equilibrium constants of DNA adsorbing to E. coli from electrochemical method and surface plasmon resonance spectroscopy, were (5.596 ± 0.462) × 105 L mol−1 and (1.24 ± 0.361) × 105 L mol−1, accordingly. Importantly, this is the first study that used an electrochemical method to express the adsorptive action of DNA by E. coli.
Keywords :
Adsorption , DNA , Escherichia coli , Electrochemistry , surface plasmon resonance
