Author/Authors :
Babri، Shirin نويسنده Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , , Mehrvash، Faezeh نويسنده Neuroscience Research Center of Tabriz University of Medical Sciences (NSRC), Tabriz, 51666-14766, Iran. , , Mohaddes، Gisou نويسنده Department of Physiology, Tabriz University of Medical Sciences, Tabriz, IR Iran , , Hatami، Homeira نويسنده Department of Biology, Faculty of Science, University of Tabriz, Tabriz, 51666-14761, Iran. , , Mirzaie، Fariba نويسنده Neuroscience Research Center of Tabriz University of Medical Sciences (NSRC), Tabriz, 51666-14766, Iran. ,
Abstract :
Purpose: The purpose of this study was to evaluate the effect of intrahippocampal injection of vitamin C and progesterone, alone or in combination, on passive avoidance learning (PAL) in multiple sclerosis.
Methods: Sixty- three male wistar rats were divided into nine groups (n=7) as following: control (saline), lesion, vitamin C (0.2, 1, 5 mg/kg), progesterone (0.01, 0.1, 1 ?g/?l) and combination therapy. Lesion was induced by intrahippocampal injection of ethidium bromide. In combination therapy, animals were treated with vitamin C (5 mg/kg) plus progesterone (0.01 mg/kg). Animals in experimental groups received different treatments for 7 days, and then all groups were tested for step through latency (STL).
Results: Our results showed that intrahippocampal injection of ethidium bromide destroys PAL significantly (p < 0.001). Treatment with vitamin C (5mg/kg) significantly (p < 0.05) improved PAL. Lower doses of progesterone did not affect latency but dose of 1 ?g/?l significantly (p < 0.05) increased STL. In combination therapy group STL was significantly (p < 0.05) more than in the lesion group, although it was not significantly different from the vitamin C group.
Conclusion: Based on our results, we concluded that intrahippocampal injection of vitamin C improves memory for PAL, but progesterone alone or in combination with vitamin C had no improving effects on memory.
