Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg+ and sIg− enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg+ and sIg− enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes.