Title of article :
Specific identification of certain probiotic Lactobacillus rhamnosus strains with PCR primers based on phage-related sequences
Author/Authors :
Brandt، نويسنده , , Kirsi and Alatossava، نويسنده , , Tapani، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Abstract :
PCR primers derived from Lactobacillus rhamnosus phage Lc-Nu genome were used to screen the presence of phage-related sequences in Lb. rhamnosus strains. Several primer pairs derived from structural and replication gene regions of phage Lc-Nu amplified PCR products of expected sizes from bacterial strains revealing phage-related sequences in 10 of 11 Lb. rhamnosus strains. Strain-specific PCR primers for three probiotic Lb. rhamnosus strains were derived from these phage-related sequences for identification and detection purposes. Specificity of these primers was tested against 11 Lb. rhamnosus strains and over 40 other bacterial strains.
Keywords :
lactic acid bacteria , PCR identification , probiotic bacteria , Primer , Phage sequence , prophage , Lysogeny , Lactobacillus rhamnosus
Journal title :
International Journal of Food Microbiology
Journal title :
International Journal of Food Microbiology