Title of article :
Purification and characterization of an antimicrobial histone H1-like protein and its gene from the testes of olive flounder, Paralichthys olivaceus
Author/Authors :
Nam، نويسنده , , Bo-Hye and Seo، نويسنده , , Jung-Kil and Go، نويسنده , , Hye-Jin and Lee، نويسنده , , Min-Jeong and Kim، نويسنده , , Young Ok and Kim، نويسنده , , Dong-Gyun and Lee، نويسنده , , Sang-Jun and Park، نويسنده , , Nam Gyu، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Abstract :
An approximately 21 kDa antimicrobial protein was purified from an acidified testis extract of olive flounder, Paralichthys olivaceus, by ion-exchange and C18 reversed-phase HPLC. A comparison of the N-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone H1 molecules; thus, it was designated flounder histone H1-like protein (fH1LP). fH1LP showed potent antimicrobial activity against Gram-positive bacteria, including Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 2.8–30.0 μg/ml), Gram-negative bacteria, including Aeromonas hydrophila, Escherichia coli D31, Vibrio parahaemolyticus (MECs, 1.4–12.0 μg/ml), and Candida albicans (MEC, 2.0 μg/ml). cDNA cloning and tissue distribution studies of fH1LP indicated that it is constitutively expressed in testis and ovary. The fH1LP expression level was significantly dependent on developmental stage, and decreased dramatically after hatching. However, lipopolysaccharide stimulation did not induce fH1LP mRNA in other immune organs, including the kidney and spleen. These results suggest that fH1LP plays an important role in innate immunity in fish during reproduction, including mating, fertilization, and hatching.
Keywords :
Testis , Olive flounder (Paralichthys olivaceus) , antimicrobial protein , Histone H1-like protein , MEC
Journal title :
Fish and Shellfish Immunology
Journal title :
Fish and Shellfish Immunology