Title of article :
Molecular characterization and functional analysis of Cactin gene from Litopenaeus vannamei
Author/Authors :
Zhang، نويسنده , , Shuang and Shi، نويسنده , , Lili and Yang، نويسنده , , Qi-Hui and Dong، نويسنده , , Xiaohui and Chi، نويسنده , , Shuyan and Liu، نويسنده , , Hong-Yu and Tan، نويسنده , , Bei-Ping، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
10
From page :
608
To page :
617
Abstract :
Cactin (Cactus interactor) is a conserved protein which was initially discovered as a novel interactor of Drosophila IkB protein Cactus. Cactin was always characterized as a negative regulator of many different developmental processes, but only found to play an immune role in humans. To better know the immune function of Cactin gene, Litopenaeus vannamei Cactin (LvCactin) was identified and characterized in this study. The full-length cDNA of LvCactin is 3, 150 bp long, with an open reading frame (ORF) encoding a Cactin_mid domain in the N-terminus 356–547 residues and a CactinC_cactus domain in the C-terminal 731–855 residues. The LvCactin protein was located in the cytoplasm and LvCactin mRNA was constitutively expressed in healthy L. vannamei, with the highest expression level in the eyestalk. LvCactin could be regulated in hemocytes after lipopolysaccharide (LPS), poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV) challenges. Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvCactin inhibited the promoters of Drosophila and shrimp antimicrobial peptide (AMP) genes. Knockdown of LvCactin by RNA interference (RNAi) increased the expression of shrimp AMP genes PEN4, crustin and ALF2 but not Lyz2. However, the mortality rates of LvCactin-knockdown shrimp in response to V. parahaemolyticus or WSSV infections were not significantly different from those of the control group. Taken together, all the results suggested that LvCactin may play a role in innate immune in L. vannamei.
Keywords :
LvCactin , Litopenaeus vannamei , Toll signal pathway , Antimicrobial peptide genes , RNA interference
Journal title :
Fish and Shellfish Immunology
Serial Year :
2014
Journal title :
Fish and Shellfish Immunology
Record number :
2113614
Link To Document :
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