Self-organized functional lipid vesicle array for sensitive immunoassay chip

We report the self-assembly immobilization of functional lipid vesicles (FLVs) by electrostatic interaction onto N-inscription-nanosized geometrics. The well-organized three-dimensional physical structures of liposome were observed by AFM. Generally, two involved forces for the binding to surfaces and the repulsion between individual liposome are necessary to array lipid vesicles individually similar to the physical configuration in solution. The immobilized FLVs demonstrated clearly defined redox activity in electrochemical measurements. We observed a notable current decrease, indicating the binding of the capture antibody with the target human serum albumin (HSA) antigen. We believe these findings can be related to various vesicles applications such as drug delivery system, nanobiosensors and nano-scale membrane function studies.