Title of article :
Same-vessel enzymatic saccharification and fermentation of organosolv/H2O2 pretreated oil palm (Elaeis guineensis Jacq.) fronds for bioethanol production: Optimization of process parameters
Author/Authors :
Cynthia Ofori-Boateng، نويسنده , , Cynthia and Lee، نويسنده , , Keat Teong، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
10
From page :
421
To page :
430
Abstract :
Based on optimized pretreatment process, oil palm fronds (OPFs) were sequentially pretreated with 1.4% (w/v) aq. NaOH in 80% ethanol with ultrasound assistance (at 75 °C for 30 min) and 3% (v/v) aq. H2O2. Using the Box–Behnken design (BBD) of response surface methodology (RSM), bioethanol production from the sono-assisted organosolv/H2O2 OPFs were optimized using same-vessel enzymatic saccharification and fermentation (SVSF) where both the hydrolysis and fermentation processes were carried out in one vessel simultaneously. Throughout the SVSF process, the incubation time and enzyme loading were kept at 72 h and 15 filter paper unit (FPU)/g substrate respectively. The other SVSF parameters which affect bioethanol yield such as temperature (X1: 30–50 °C), solid loading (X2: 5.0–10.0% w/v), yeast concentration (X3: 5.0–20 g/l) and pH (X4: 4.0–7.0) were optimized. Well fitted regression equations (R2 > 0.97) obtained were able to predict reliable optimum bioethanol concentration and yield. The predicted optimum bioethanol concentration (i.e., 20.61 g/l) and yield (i.e., 84.60%) were attained at 36.94 °C (∼37 °C), 7.57% w/v solid loading (∼8.0% w/v), 13.97 g/l yeast concentration (∼14.0 g/l) and pH of 5.29 (∼5.30). Validated results indicated a maximum ethanol concentration and yield of 21.96 g/l and 84.65% respectively, which were closer to the predicted optimum responses. Using the optimum conditions, the highest bioethanol productivity of 0.76 g/l/h was observed at 12 h of SVSF process.
Keywords :
Same-vessel saccharification and fermentation , optimization , Organosolv pretreatment , Bioethanol , Oil palm fronds
Journal title :
Energy Conversion and Management
Serial Year :
2014
Journal title :
Energy Conversion and Management
Record number :
2337413
Link To Document :
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