Saliva Components Reestablish the Basal Production of IL-6 by Mononuclear cells, 72 Hours after Nitinol Archiwire Placement: A Preliminary Study

The purpose of the study was to evaluate interleukin-6 production, in saliva-activated mononuclear cell cultures from malocclusion patients, before and after placement of .014 NiTi archwires. Four patients receiving .014 Nitinol archwire to correct malocclusion participated in this study. Samples of their blood and saliva were collected before and after placement of the apparatus. Mononuclear cells were obtained from the blood using the Ficoll-Paque (1.077 g/ml) density gradient separation method. Mononuclear Cells were activated with saliva from each patient and were cultured in 96-well plates for 72 hours. Samples were collected at 24 hours before apparatus placement, and at 24 hours and 72 hours after placement. IL-6 expression levels in the cell culture supernatants were quantified by ELISA. An increase in IL-6 levels in the cell culture supernatants was observed 24 hours after placement of the orthodontic apparatus relative to the negative control (p= 0.002) and IL-6 came to basal limits 72 hours after apparatus placement. IL-6 quantification may be useful as a biomarker to estimate the inflammatory response caused by forces applied during orthodontic treatment and their levels came to basal limits 72 hours after apparatus placement in patients without systemic diseases. The isolation of saliva components involved in such effects is important to study the mechanisms to control the acute inflammation in oral cavity after apparatus placement.