Author/Authors :
Yazdian، Shabnam نويسنده Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Yazdian, Shabnam , Fahham، Najmeh نويسنده Department of Pharmacology, Iran Pasteur Institute, Tehran, Iran. Fahham, Najmeh , Ghahremani ، Mohammad Hossein نويسنده Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran , , Afzali، Monireh نويسنده Virtual School, Tehran University of Medical Sciences, Tehran, Iran. Afzali, Monireh , Farsandaj، Narges نويسنده Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Farsandaj, Narges , Vatankhah، Melody نويسنده Department of Biology, College of Science, University of Tehran, Tehran, Iran. Vatankhah, Melody , Ostad، Seyed Nasser نويسنده ,
Abstract :
There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells. Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.
