Author/Authors :
Mostafavinia، Seyedeh Elham نويسنده Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, IR Iran , , Hoshyar، Reyhane نويسنده Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, IR Iran ,
Abstract :
Some herbal anticancer agents have direct interactions with DNA. The present study aimed to investigate the interaction of ctDNA with rosemary flowers. We used UV-Vis, fluorescence and circular dichroism (CD) spectroscopic techniques. The absorption of DNA at 260 nm increased on addition of rosemary. Herb extract quench the fluorescence of EtBr bound to ctDNA. The Stern-Volmer constant (K) value for rosemary extract is 0.39 (mg/mL)-1. Finally, the CD spectra indicate that rosemary induces some conformational changes in ctDNA structure such as B to C-form transition. Our results illustrate that rosemary extracts interact with ctDNA through minor groove binding. This is one of the molecular mechanisms underlying anticancer effects of rosemary.
