Author/Authors :
Mohammadi, Mojgan Departments of Immunology - Department of Immunology - The Faculty of Life Sciences - Michael Smith Building , Bazrafshani, Mohammad Reza Medical Genetics - Kerman University of Medical Sciences - Centre for Integrated Genomic Medical Research (CIGMR) - Stopford Building - Oxford Road - Manchester - M13 9PT, United Kingdom , Day, Philip.J Centre for Integrated Genomic Medical Research (CIGMR) - Stopford Building - Oxford Road - Manchester - M13 9PT, United Kingdom , Ollier, William. E.R Centre for Integrated Genomic Medical Research (CIGMR) - Stopford Building - Oxford Road - Manchester - M13 9PT, United Kingdom
Abstract :
Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF –1154 G and –2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF poly-morphisms and the influence of TNF-α and IL-4 on VEGF production was studied. Methods: VEGF –1154 G/A and –2578 C/A were detected using ARMS-PCR. To de-termine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA. Re-sults: The combinations of –1154 GG/-2578 CC and –1154 GG/-2578 CA were signifi-cantly associated with higher VEGF production (p<0.0001). Production of VEGF was significantly influenced by TNF-α in individuals who had certain VEGF genotype com-binations. Although VEGF production was dramatically suppressed by IL-4, it was not dependent on VEGF genotype. Conclusions: Since TNF-α has influence on the graft outcome, to avoid allocation of grafts from high TNF-α producer donors to recipients, it might be useful to predict and minimize graft rejection by having prior knowledge of TNF-α and also VEGF genotypes especially -1154 G/A and -2578 C/A VEGF.
